mouse anti e2f3  (Santa Cruz Biotechnology)

Journal: Cell & Bioscience

Article Title: IgD in nucleus of pro-B cells promotes pro-B cells proliferation by regulating E2F3 expression

doi: 10.1186/s13578-025-01490-y

Figure Lengend Snippet: IgD deletion reduces the accessibility of E2f3a gene to inhibit E2f3a expression. A A heatmap representation of ChIP-seq data of transcription factors (TFs) was utilized to evaluate the genomic occupancy of IgD. B Genome browser tracks illustrate the promoter region at the E2f3 target site in pro-B cells, comparing conditions with anti-IgD to those with an isotype IgG control. C EMSA was used to confirm the specific binding of IgD to the E2f3 promoter region, with biotin-labeled sequences of the E2f3 promoter region used as probes. The assay included a non-biotinylated competition probe (Com Probe), representing an unlabeled sequence of the E2f3 promoter, and a mutation probe (Mut Probe) which has identical sequence with targeted nucleotide substitutions in the core E2F binding motif. The reaction system is depicted in the figure. D Supershift assay detects the specific binding of IgD to the E2f3 promoter region, the reaction system is as shown in the figure. E ChIP-PCR was performed to amplify the promoter regions of E2f3a and E2f3b using pro-B cells from WT mice, with genomic DNA from pro-B cells serving as a positive control. F Transcriptional levels of E2F3a and E2F3b in pro-B cell and pre-B cell derived from IgD −/− mice were detected by RT-PCR. G Protein expression levels of E2f3a in pro-B cell from WT and IgD −/− mice were examined by Western blot analysis. Quantification result was presented as grey scale scans with statistical significance denoted by asterisks (**** p < 0.0001)

Article Snippet: Primary antibodies used included anti-mouse β-actin (Zhongshan Golden Bridge, TA-09), anti-mouse Gapdh (Zhongshan Golden Bridge, TA-08), anti-mouse IgD (Santa, sc-53853), anti-mouse IgM (Bethyl, A90-101P), anti-mouse Igκ (SouthernBiotech, 1050-08), and anti-mouse E2f3 (CST, DF12390).

Techniques: Expressing, ChIP-sequencing, Control, Binding Assay, Labeling, Sequencing, Mutagenesis, Positive Control, Derivative Assay, Reverse Transcription Polymerase Chain Reaction, Western Blot

Journal: Virus research

Article Title: Possible etiological association of ovine papillomaviruses with bladder tumors in cattle.

doi: 10.1016/j.virusres.2023.199084

Figure Lengend Snippet: Fig. 5. E2F3 protein expression in bladders of cattle. (A) WB analysis of E2F3 in four healthy and 10 numbered bladder tumors. (B) Densitometric analysis of E2F3 protein was performed relative with the GAPDH protein levels (*p ≤0.05; **p ≤0.01). Plots represent value found in each sample performed in duplicate.

Article Snippet: Rabbit polyclonal anti-PDGFβR and antiphosphorylated PDGFβR antibodies, as well as mouse antibodies against E2F3 and GAPDH, were purchased from Santa Cruz Biotechnology (TX, USA).

Techniques: Expressing

Journal: Molecular medicine reports

Article Title: MicroRNA‑433 reduces cell proliferation and invasion in non‑small cell lung cancer via directly targeting E2F transcription factor 3.

doi: 10.3892/mmr.2018.9020

Figure Lengend Snippet: Figure 3. E2F3 is a direct target of miR‑433 in non‑small‑cell lung cancer. (A) Putative Wt and Mut binding sequences in the 3'‑UTR of E2F3. (B) A549 and H460 cells were cotransfected with miR‑433 mimics or miR‑NC and pMIR‑Wt‑E2F3‑3'‑UTR or pMIR‑Mut‑E2F3‑3'‑UTR. Following transfection for 48 h, relative luciferase activity was determined using a dual luciferase reporter assay system. *P<0.05 vs. miR‑NC. E2F3 (C) mRNA and (D) protein expression levels were detected by reverse transcription‑quantitative polymerase chain reaction and western blot analysis, respectively, in A549 and H460 cells transfected with miR‑433 mimics or miR‑NC. *P<0.05 vs. miR‑NC. E2F3, E2F transcription factor 3; miR, microRNA; Mut, mutant; NC, negative control; pMIR, plasmid vector; UTR, untranslated region; Wt, wild‑type.

Article Snippet: The primary antibodies used in the present study were acquired from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA) and included mouse anti-human monoclonal E2F3 (1:1,000; cat. no. sc-28308) and mouse anti-human monoclonal GAPDH (1:1,000; cat. no. sc-365062) antibodies.

Techniques: Binding Assay, Transfection, Luciferase, Activity Assay, Reporter Assay, Expressing, Polymerase Chain Reaction, Western Blot, Mutagenesis, Negative Control, Plasmid Preparation

Journal: Molecular medicine reports

Article Title: MicroRNA‑433 reduces cell proliferation and invasion in non‑small cell lung cancer via directly targeting E2F transcription factor 3.

doi: 10.3892/mmr.2018.9020

Figure Lengend Snippet: Figure 4. E2F3 downregulation suppresses cell proliferation and invasion in non‑small‑cell lung cancer. (A) E2F3 siRNA or NC siRNA was transfected into A549 and H460 cells. A total of 72 h post‑transfection, western blot analysis was performed to detect E2F3 protein expression levels. *P<0.05 vs. NC siRNA. The effect of E2F3 knockdown on A549 and H460 cell proliferation and invasion was determined by a (B) Cell Counting kit‑8 and (C) cell invasion assays, respectively (magnification, x200). *P<0.05 vs. NC siRNA. E2F3, E2F transcription factor 3; NC, negative control; OD, optical density; siRNA, small interfering RNA.

Article Snippet: The primary antibodies used in the present study were acquired from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA) and included mouse anti-human monoclonal E2F3 (1:1,000; cat. no. sc-28308) and mouse anti-human monoclonal GAPDH (1:1,000; cat. no. sc-365062) antibodies.

Techniques: Transfection, Western Blot, Expressing, Knockdown, CCK-8 Assay, Negative Control, Small Interfering RNA

Journal: Molecular medicine reports

Article Title: MicroRNA‑433 reduces cell proliferation and invasion in non‑small cell lung cancer via directly targeting E2F transcription factor 3.

doi: 10.3892/mmr.2018.9020

Figure Lengend Snippet: Figure 5. E2F3 restoration counteracts the effects of miR‑433 on non‑small‑cell lung cancer cell proliferation and invasion. A549 and H460 cells were trans fected with miR‑433 mimics with pcDNA3.1‑E2F3 or pcDNA3.1, or miR‑NC alone. (A) Western blot analysis was employed to detect E2F3 protein expression levels at 72 h following transfection. *P<0.05 vs. miR‑NC. #P<0.05 vs. miR‑433 mimics+pcDNA3.1‑E2F3. (B) Cell Counting kit‑8 and (C) cell invasion assays were applied to determine the proliferation and invasion in variably treated cells. *P<0.05 vs. miR‑NC. #P<0.05 vs. miR‑433 mimics+pcDNA3.1‑E2F3 (magnification, x200). E2F3, E2F transcription factor 3; miR, microRNA; NC, negative control; pcDNA3.1, plasmid vector; OD, optical density.

Article Snippet: The primary antibodies used in the present study were acquired from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA) and included mouse anti-human monoclonal E2F3 (1:1,000; cat. no. sc-28308) and mouse anti-human monoclonal GAPDH (1:1,000; cat. no. sc-365062) antibodies.

Techniques: Western Blot, Expressing, Transfection, CCK-8 Assay, Negative Control, Plasmid Preparation